RESUMO
BACKGROUND: Angiotensin-converting enzyme 2 (ACE2) has a protective role in the regulation of blood pressure. AIM: To evaluate the influence of ACE2 rs1978124 gene polymorphism on the hemodynamic response after a six-minute walk submaximal aerobic test. MATERIAL AND METHODS: A six-minute walk submaximal aerobic test was carried out in 47 men and 55 women, aged 22 ± 2 years. Blood pressure was recorded before and after the test. Pulse rate was recorded continuously. ACE2 polymorphism was determined in DNA extracted from a blood sample. RESULTS: For women, the genotype distribution did not deviate from Hardy-Weinberg equilibrium (x2 = 0.804, d.f. = 1, P = 0.4) and no significant differences in heart rate, systolic and diastolic blood pressure were observed between CC, CT and TT genotypes before and after the test. Among men no differences in these parameters were observed either between the three phenotypes. CONCLUSIONS: ACE2 rs1978124 gene polymorphism did not influence the hemodynamic response to submaximal exercise in these participants.
Assuntos
Enzima de Conversão de Angiotensina 2 , Exercício Físico , Enzima de Conversão de Angiotensina 2/genética , Pressão Sanguínea , Feminino , Genótipo , Hemodinâmica , Humanos , Masculino , Polimorfismo Genético , Adulto JovemRESUMO
Background: Angiotensin-converting enzyme 2 (ACE2) has a protective role in the regulation of blood pressure. Aim: To evaluate the influence of ACE2 rs1978124 gene polymorphism on the hemodynamic response after a six-minute walk submaximal aerobic test. Material and Methods: A six-minute walk submaximal aerobic test was carried out in 47 men and 55 women, aged 22 ± 2 years. Blood pressure was recorded before and after the test. Pulse rate was recorded continuously. ACE2 polymorphism was determined in DNA extracted from a blood sample. Results: For women, the genotype distribution did not deviate from Hardy-Weinberg equilibrium (x2 = 0.804, d.f. = 1, P = 0.4) and no significant differences in heart rate, systolic and diastolic blood pressure were observed between CC, CT and TT genotypes before and after the test. Among men no differences in these parameters were observed either between the three phenotypes. Conclusions: ACE2 rs1978124 gene polymorphism did not influence the hemodynamic response to submaximal exercise in these participants.
Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Exercício Físico , Enzima de Conversão de Angiotensina 2/genética , Polimorfismo Genético , Pressão Sanguínea , Genótipo , HemodinâmicaRESUMO
Determinar la distribución genotípica y la frecuencia alélica del polimorfismo rs17817449 del gen FTO en jóvenes chilenos y su influencia en variables antropométricas. Los 96 sujetos jóvenes (18-25 años), 43 hombres y 53 mujeres fueron evaluados utilizando genotipificación del polimorfismo rs17817449 del gen FTO en TT, TG y GG mediante polimerase chain reaction (PCR), además de una evaluación Kinenatropométrica para determinar las variables asociadas a composición corporal. Las variables fueron analizadas estadísticamente según su distribución paramétrica y el nivel de significancia estadística fue p<0,05. La distribución genotípica del polimorfismo rs17817449 de FTO en jóvenes chilenos fue: TT: 50 %; TG: 42,7 %; GG 7,3 % y la distribución alélica fue: T: 0,7105 y G: 0,2895. No se encontraron diferencias estadísticamente significativas en las variables antropométricas al analizar los participantes según modelo de dominancia del alelo G. Se determinó la distribución genotípica y la frecuencia alélica del polimorfismo rs17817449 del gen FTO en jóvenes chilenos, datos desconocidos hasta este momento. De acuerdo a nuestros resultados, no existen diferencias antropométricas entre personas con diferentes genotipos del polimorfismo rs17817449 de FTO, agrupadas según modelo de dominancia del alelo G.
The rs17817449 polymorphism of the FTO gene in young Chileans and their influence on anthropometric variables. 96 young subjects (18-25 years old), 43 men and 53 women were evaluated using genotyping of the rs17817449 polymorphism of the FTO gene in TT, TG and GG by means of polymerase chain reaction (PCR), in addition to a Kinenatropometric evaluation to determine the variables associated with body composition. The variables were analyzed statistically according to their parametric distribution and the level of statistical significance was p<0.05. The genotypic distribution of the FTO polymorphism rs17817449 in young Chileans was: TT: 50 %; TG: 42.7 %; GG 7.3 % and the allelic distribution was: T: 0.7105 and G: 0.2895. No statistically significant differences were found in the anthropometric variables when analyzing the participants according to model of dominance of the G allele. The genotypic distribution and the allelic frequency of the rs17817449 polymorphism of the gene were determined FTO in Chilean population, data unknown until now. According to our results, there are no anthropometric differences between people with different genotypes of the FTO polymorphism rs17817449, nor according to the dominance model of the G.
Assuntos
Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Antropometria , Polimorfismo de Nucleotídeo Único , Dioxigenase FTO Dependente de alfa-Cetoglutarato , Variação Genética , Índice de Massa Corporal , Chile , Reação em Cadeia da Polimerase , Adiposidade/genética , Circunferência da Cintura/genética , Frequência do Gene , GenótipoRESUMO
RESUMEN: La expresión de los genes puede impactar sobre el rendimiento muscular. En este aspecto el polimorfismo del gen de la Enzima Convertidora de Angiotensina (ECA), dependiendo de su inserción (Ins) o deleción (Del) puede potenciar diferentes cualidades musculares. Del mismo modo, si consideramos la ventilación como un proceso vital, sería relevante investigar si existe una influencia de este polimorfismo sobre los músculos que llevan a cabo tan importante función. El objetivo de este trabajo consistió en determinar el rendimiento muscular inspiratorio y espiratorio según alelos y polimorfismos del gen de la ECA. Se sometió a 83 sujetos (18 a 35 años), 46 hombres y 37 mujeres, a evaluaciones de capacidad vital forzada (CVF), presión inspiratoria máxima y presión espiratoria máxima (PIM-PEM). Posterior a esto, la genotipificación fue realizada por polimerase chain reaction (PCR) y electroforesis en gel de agarosa. Las variables fueron sometidas al análisis estadístico correspondiente según su distribución paramétrica, el nivel de significancia establecido fue un p<0,05. La distribución genotípica fue: Ins/Ins: 28 %, Ins/Del: 62 % y Del/Del: 19 %. Las mujeres homocigoto Ins, presentaron mayor PIM y PEM en modelos por genotipos (p=0,043; p=0,0001 respectivamente) y por dominancia (p=0,019; p=0,0008 respectivamente). La distribución genotípica y frecuencia alélica fue similar a la descrita anteriormente en población chilena. Además, las mujeres portadoras del alelo Ins, presentaron mayor PIM y PEM.
SUMMARY: Gene expression can impact muscle performance. In this aspect genetic polymorphism of the Angiotensin Converting Enzyme (ACE), depending on its insertion or deletion can strengthen different muscle qualities. Similarly, if we consider ventilation as a vital process, it would be important to research whether or not, there is an influence of this polymorphism on the muscles that perform such an important function. The aim of this study was to determine the inspiratory and expiratory muscle performance according alleles and polymorphisms of the ACE gene. We subjected 83 individuals (18-35 years), 46 men and 37 women, to forced vital capacity evaluations, maximum inspiratory pressure and expiratory pressure. Genotyping was subsequently performed by polymerase chain reaction (PCR) and agarose gel electrophoresis. The variables were subjected to appropriate statistical analysis by parametric distribution, the significance level was set at p <0.05. The genotype distribution was: Ins/Ins: 28 %, Ins/Del: 62 % and Del/Del: 19 %. Women homozygous Ins, exhibited a higher maximum inspiratory pressure and expiratory pressure in models for genotypes (p = 0.043; p = 0.0001 respectively) and for dominance (p = 0.019; p = 0.0008 respectively). The genotype distribution and allele frequency was similar to that described above, in Chilean population. Furthermore, women carrying the Ins allele had a higher maximum inspiratory pressure and expiratory pressure.
